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Title:Vpliv v gojišče dodanega meta-topolina na rast in razraščanje marelice (Prunus armeniaca L.) v tkivnih kulturah
Authors:ID Hlebš, Lana (Author)
ID Šiško, Metka (Mentor) More about this mentor... New window
Files:.pdf VS_Hlebs_Lana_2019.pdf (647,48 KB)
MD5: 83A6D3F963A8177B4EBEFA4497BA116C
PID: 20.500.12556/dkum/8f8c9839-fbfd-4441-889d-63b49da86f7b
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:Na Fakulteti za kmetijstvo in biosistemske vede smo v letu 2019 proučevali vpliv dodanega meta-topolina v rastno gojišče na rast in razraščanje marelice v tkivnih kulturah. Za rastlinski material smo uporabili lokalni genotip marelice, poznan pod imenom Pišečka marelica, ki je že bila iniciirana v tkivne kulture. Marelico smo razmnoževali na osnovnem gojišču McCown Woody Plant, ki smo mu dodali različne koncentracije meta-topolina (mT) ter 1-naftilocetne kisline (NAA). Pripravili smo kontrolno gojišče (G0), ki ni vsebovalo rastnih hromonov, gojišče G1, ki je vseboval 0,5 mg/l mT in 0,01 mg/l NAA ter gojišče G2, ki je vsebovalo 1,0 mg/l mT in 0,01 mg/l NAA. Opazovali smo povečanje prirasta mase, število poganjkov ter delež vitrifikacije pri rastlinah na posameznem gojišču. Ugotovili smo, da se je najbolje izkazalo gojišče G2, na katerem smo zabeležili povprečni 19,5 faktorski prirast oz. 0,372 g sveže mase, 4,5 poganjka na rastlino, noben poganjek ni bil vitrificiran. Pri gojišču G1 smo opazili 17,9 faktorski prirast oz. 0,326 g povprečne mase, 4,2 poganjka ter en vitrificiran poganjek, medtem ko so bile vrednosti pri kontrolnem gojišču značilno nižje – 3,8 faktorski prirast oz. 0,071 g mase, 1,22 poganjka in trije primeri vitrifikacije. S statistično obdelavo podatkov smo ugotovili tudi korelacijo med prirastom mase rastlin ter številom poganjkov (r = 0,85) in korelacijo med faktorskim povečanjem koncentracije hormonov v gojišču in prirastom mase rastlin (r = 0,63) ter števila poganjkov (r = 0,61).
Keywords:marelica, Prunus armeniaca L., tkivne kulture, prirast, poganjki
Place of publishing:Maribor
Year of publishing:2019
PID:20.500.12556/DKUM-73835 New window
NUK URN:URN:SI:UM:DK:9NAJOTED
Publication date in DKUM:28.06.2019
Views:1410
Downloads:207
Metadata:XML DC-XML DC-RDF
Categories:FKBV
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Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:19.06.2019

Secondary language

Language:English
Title:Effect of meta-Topolin added to the medium on growth and shoot formation of apricot (Prunus armeniaca L.) in tissue culture
Abstract:In 2019, an experiment was conducted at the Faculty of Agriculture and Life Sciences of the University of Maribor, where the effect of meta-Topolin added to the medium on growth and branching of apricots was studied. The apricot local varietal material Pišečka that was used in this experiment, was already introduced to the tissue culture. Increase of plantlet weight, number of shoots and rate of vitrification in the media with different concentrations of plant growth hormones were observed. The McCown Woody Plant was used as the growth medium, to which different concentrations of meta-topolin (mT) and 1-naphthaleneacetic acid (NAA) were added. The growth media were divided into 3 groups: the control medium G0, which did not contain growth regulators, the medium G1, to which 0.5 mg/l mT and 0.01 mg/l NAA were added, and the medium G2 which contained 1.0 mg/l mT in 0.01 mg/l NAA. The medium G2 produced the best results with a factor of 19.5 or 0.372 g plantlet weight increase, 4.5 shoots per plantlet and zero vitrification rate. In G1 medium a factor of 17.9 or 0.326 g weight increase, 4.2 shoots per plantlet and one case of vitrification were documented. As for the control medium, a significantly lower success was obtained. The values of the control medium were 3,8 factorial increase or 0.071 g plantlet weight increase, 1.22 shoots per plantlet and no vitrification noted in any of plantlets. A positive correlation between plantlet weight increase and number of shoots was determined (r = 0.85). Futhermore, correlations between the factorial increase of hormones in the medium and weight increase (r = 0.63), along with number of shoots per plantlet (r = 0.61) were perceived.
Keywords:apricot, Prunus armeniaca L., tissue culture, plant weight, shoots


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