| Title: | Efficient and Green Isolation of Keratin from Poultry Feathers by Subcritical Water |
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| Authors: | ID Škerget, Mojca, Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia (Author)
ID Čolnik, Maja, Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia (Author)
ID Zemljič, Lidija Fras, Laboratory for Characterization and Processing of Polymers, Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia (Author)
ID Gradišnik, Lidija, Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, Taborska 8, 2000 Maribor, Slovenia (Author)
ID Semren, Tanja Živković, Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Ksaverska Cesta 2, 10000 Zagreb, Croatia (Author)
ID Lovaković, Blanka Tariba, Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Ksaverska Cesta 2, 10000 Zagreb, Croatia (Author)
ID Maver, Uroš, Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, Taborska 8, 2000 Maribor, Slovenia Department of Pharmacology, Faculty of Medicine, University of Maribor, Taborska 8, 2000 Maribor, Slovenia (Author) |
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| Files: |  polymers-15-02658-v2.pdf (2,78 MB)
MD5: ADC560177A388899C11397A9713070BF
 https://www.mdpi.com/2073-4360/15/12/2658
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | The isolation of keratin from poultry feathers using subcritical water was studied in a batch reactor at temperatures (120–250 °C) and reaction times (5–75 min). The hydrolyzed product was characterized by FTIR and elemental analysis, while the molecular weight of the isolated product was determined by SDS-PAGE electrophoresis. To determine whether disulfide bond cleavage was followed by depolymerization of protein molecules to amino acids, the concentration of 27 amino acids in the hydrolysate was analyzed by GC/MS. The optimal operating parameters for obtaining a high molecular weight protein hydrolysate from poultry feathers were 180 °C and 60 min. The molecular weight of the protein hydrolysate obtained under optimal conditions ranged from 4.5 to 12 kDa, and the content of amino acids in the dried product was low (2.53% w/w). Elemental and FTIR analyses of unprocessed feathers and dried hydrolysate obtained under optimal conditions showed no significant differences in protein content and structure. Obtained hydrolysate is a colloidal solution with a tendency for particle agglomeration. Finally, a positive influence on skin fibroblast viability was observed for the hydrolysate obtained under optimal processing conditions for concentrations below 6.25 mg/mL, which makes the product interesting for various biomedical applications. |
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| Keywords: | poultry feathers, subcritical water hydrolysis, keratin, physico-chemical characterization, cytotoxicity |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 16.05.2023 |
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| Article acceptance date: | 09.06.2023 |
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| Publication date: | 12.06.2023 |
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| Publisher: | MDPI |
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| Year of publishing: | 2023 |
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| Number of pages: | str. 2658 |
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| Numbering: | Vol. 15, no. 12 |
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| PID: | 20.500.12556/DKUM-86411  |
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| UDC: | 66.02 |
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| ISSN on article: | 2073-4360 |
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| eISSN: | 2073-4360 |
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| COBISS.SI-ID: | 155799299 |
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| DOI: | 10.3390/polym15122658 |
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| Copyright: | © 2023 by the authors |
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| Publication date in DKUM: | 28.11.2023 |
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| Views: | 648 |
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| Downloads: | 197 |
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| Metadata: |    |
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| Categories: | Misc.
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