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Title:Vpliv različnih načinov sterilizacije in tipa brstov na uspešno vzpostavitev tkivne kulture pri navadni bodiki ( Ilex aquifolium L.) : diplomsko delo
Authors:ID Modrič, Urška (Author)
ID Šiško, Metka (Mentor) More about this mentor... New window
ID Ivanuš, Anja (Comentor)
Files:.pdf VS_Modric_Urska_2023.pdf (1,45 MB)
MD5: 17A45231802998FB39CF51B388F33A88
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:V letu 2023 smo na Fakulteti za kmetijstvo in biosistemske vede v Mariboru opravili poskus, v katerem smo proučevali vpliv različnih načinov sterilizacije in vpliv tipa brstov (apikalni in aksilarni) na vzpostavitev tkivne kulture navadne bodike (Ilex aquifolium L.). Za razkuževanje smo uporabili dve različni sterilizacijski sredstvi, dikloroizocianurno kislino (DICA) in natrijev hipoklorit (NaClO), v različnih koncentracijah in z različnim časom tretiranja. V raziskavo je bilo vključenih 640 brstov, na katerih smo skupno opravili 7 različnih načinov sterilizacije. Vse brste smo predhodno tretirali v 70-% etanolu za 40 sekund. Razkužene brste smo prenesli na osnovno gojišče McCown Woody Plant in spremljali rast. Ugotovili smo, da tako način sterilizacije kot tudi tip brstov vplivata na pridobljeno število vitalnih poganjkov. Skupno smo uspešno sterilizirali 22 brstov. Za uspešnejše so se izkazali apikalni brsti. Pri primerjavi sterilizacijskih sredstev se je tretiranje z DICA izkazalo za uspešnejše, pridobili smo 18 vitalnih brstov. Najuspešnejša je bila sterilizacija brstov z DICA in časom tretiranja 5 minut, kjer smo pridobili 7 vitalnih brstov.
Keywords:sterilizacija, tkivne kulture, bodika, in vitro, brsti
Place of publishing:Maribor
Place of performance:Maribor
Publisher:U. Modrič
Year of publishing:2023
Number of pages:1 spletni vir (1 datoteka PDF (IX, 10-45, [1] str.))
PID:20.500.12556/DKUM-86559 New window
UDC:631.533.3:582.314.1:57.086.83(043.2)=163.6
COBISS.SI-ID:179955971 New window
Publication date in DKUM:08.01.2024
Views:480
Downloads:48
Metadata:XML DC-XML DC-RDF
Categories:FKBV
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:12.12.2023

Secondary language

Language:English
Title:Effect of different sterilisation techniques and common holly ( Ilex aquifilium l.) bud type on the success of establishing tissue culture
Abstract:In 2023, an experiment was conducted at the Faculty of Agriculture and Life Sciences in Maribor, to investigate the effect of different sterilisation techniques and the influence of bud type (apical and axillary) on the establishment of tissue culture of common holly (Ilex aquifolium L.). Two different sterilising agents, dichloroisocyanuric acid (DICA) and sodium hypochlorite (NaClO), were used in different concentrations and treatment times for disinfection. The study included 640 buds and seven sterilisation treatments. All buds were first treated in 70 % ethanol for 40 seconds. After disinfection, the buds were placed on McCown Woody Plant Medium and their growth was observed. The results showed that both the method of sterilisation and the type of buds influenced the number of viable shoots obtained. Of the 640 buds, only 22 were successfully sterilised, with the apical buds proved to be more successful. When comparing the sterilisation methods, the DICA treatment proved to be more successful, as 18 viable buds were obtained. The most effective sterilization method was a 5-minute treatment of the buds with DICA, which produced seven viable buds.
Keywords:sterilisation, tissue culture, holly, in vitro, buds


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