| Title: | Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytes |
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| Authors: | ID Karaffová, Viera (Author)
ID Teleky, Jana (Author)
ID Kozmos, Maša (Author)
ID Langerholc, Tomaž (Author)
ID Mudroňová, Dagmar (Author)
ID Hudec, Erik (Author)
ID Ševčíková, Zuzana (Author) |
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| Files: |  Karaffova-2023-Application_of__i_Lactobacillus.pdf (3,09 MB)
MD5: 97E67CA8B1C9FBFE0A28C4F8C1965013
 https://doi.org/10.3390/life13051090
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKBV - Faculty of Agriculture and Life Sciences
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| Abstract: | In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 x 10(7) and 1 x 10(9) CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1 beta, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 x 10(5) cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation. |
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| Keywords: | Lactobacillus reuteri B1/1, CLAB cells, pro-inflammatory cytokine, metabolic activity |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 22.03.2023 |
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| Article acceptance date: | 25.04.2023 |
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| Publication date: | 27.04.2023 |
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| Publisher: | MDPI |
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| Year of publishing: | 2023 |
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| Number of pages: | Str. 1-14 |
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| Numbering: | Letn. 13, Št. 5, št. članka 1090 |
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| PID: | 20.500.12556/DKUM-87111  |
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| UDC: | 579 |
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| ISSN on article: | 2075-1729 |
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| COBISS.SI-ID: | 150715651 |
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| DOI: | 10.3390/life13051090 |
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| Publication date in DKUM: | 20.02.2024 |
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| Views: | 446 |
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| Downloads: | 20 |
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| Metadata: |    |
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| Categories: | Misc.
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