| Naslov: | Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytes |
|---|
| Avtorji: | ID Karaffová, Viera (Avtor)
ID Teleky, Jana (Avtor)
ID Kozmos, Maša (Avtor)
ID Langerholc, Tomaž (Avtor)
ID Mudroňová, Dagmar (Avtor)
ID Hudec, Erik (Avtor)
ID Ševčíková, Zuzana (Avtor) |
|---|
| Datoteke: |  Karaffova-2023-Application_of__i_Lactobacillus.pdf (3,09 MB)
MD5: 97E67CA8B1C9FBFE0A28C4F8C1965013
 https://doi.org/10.3390/life13051090
|
|---|
| Jezik: | Angleški jezik |
|---|
| Vrsta gradiva: | Znanstveno delo |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: | FKBV - Fakulteta za kmetijstvo in biosistemske vede
|
|---|
| Opis: | In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 x 10(7) and 1 x 10(9) CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1 beta, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 x 10(5) cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation. |
|---|
| Ključne besede: | Lactobacillus reuteri B1/1, CLAB cells, pro-inflammatory cytokine, metabolic activity |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Poslano v recenzijo: | 22.03.2023 |
|---|
| Datum sprejetja članka: | 25.04.2023 |
|---|
| Datum objave: | 27.04.2023 |
|---|
| Založnik: | MDPI |
|---|
| Leto izida: | 2023 |
|---|
| Št. strani: | Str. 1-14 |
|---|
| Številčenje: | Letn. 13, Št. 5, št. članka 1090 |
|---|
| PID: | 20.500.12556/DKUM-87111  |
|---|
| UDK: | 579 |
|---|
| COBISS.SI-ID: | 150715651 |
|---|
| DOI: | 10.3390/life13051090 |
|---|
| ISSN pri članku: | 2075-1729 |
|---|
| Datum objave v DKUM: | 20.02.2024 |
|---|
| Število ogledov: | 449 |
|---|
| Število prenosov: | 20 |
|---|
| Metapodatki: |    |
|---|
| Področja: | Ostalo
|
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Skupna ocena: | (0 glasov) |
|---|
| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
|---|
| Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
