| Title: | Glucose-stimulated calcium dynamics in beta cells from male C57BL/6J, C57BL/6N, and NMRI mice : a comparison of activation, activity, and deactivation properties in tissue slices |
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| Authors: | ID Pohorec, Viljem (Author)
ID Križančić Bombek, Lidija (Author)
ID Skelin, Maša (Author)
ID Dolenšek, Jurij (Author)
ID Stožer, Andraž (Author) |
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| Files: |  Pohorec-2022-Glucose-Stimulated_Calcium_Dynami.pdf (4,45 MB)
MD5: 33BA39396E86135AD44159AE65194A16
 https://doi.org/10.3389/fendo.2022.867663
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
FNM - Faculty of Natural Sciences and Mathematics
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| Abstract: | Although mice are a very instrumental model in islet beta cell research, possible phenotypic differences between strains and substrains are largely neglected in the scientific community. In this study, we show important phenotypic differences in beta cell responses to glucose between C57BL/6J, C57BL/6N, and NMRI mice, i.e., the three most commonly used strains. High-resolution multicellular confocal imaging of beta cells in acute pancreas tissue slices was used to measure and quantitatively compare the calcium dynamics in response to a wide range of glucose concentrations. Strain- and substrain-specific features were found in all three phases of beta cell responses to glucose: a shift in the dose-response curve characterizing the delay to activation and deactivation in response to stimulus onset and termination, respectively, and distinct concentration-encoding principles during the plateau phase in terms of frequency, duration, and active time changes with increasing glucose concentrations. Our results underline the significance of carefully choosing and reporting the strain to enable comparison and increase reproducibility, emphasize the importance of analyzing a number of different beta cell physiological parameters characterizing the response to glucose, and provide a valuable standard for future studies on beta cell calcium dynamics in health and disease in tissue slices. |
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| Keywords: | beta cell, mouse models, calcium imaging, glucose-dependence, tissue slice |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 04.02.2022 |
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| Article acceptance date: | 23.02.2022 |
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| Publication date: | 24.03.2022 |
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| Publisher: | Frontiers Research Foundation |
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| Year of publishing: | 2022 |
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| Number of pages: | Str. 1-16 |
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| Numbering: | Letn. 13, št. članka 867663 |
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| PID: | 20.500.12556/DKUM-88634  |
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| UDC: | 612.349.7 |
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| ISSN on article: | 1664-2392 |
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| COBISS.SI-ID: | 103005187 |
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| DOI: | 10.3389/fendo.2022.867663 |
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| Publication date in DKUM: | 15.07.2024 |
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| Views: | 298 |
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| Downloads: | 27 |
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| Metadata: |    |
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| Categories: | Misc.
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