| Title: | Covalent modification of chitosan surfaces with a sugar amino acid and lysine analogues |
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| Authors: | ID Dorn, Tobias (Author)
ID Finšgar, Matjaž (Author)
ID Stana-Kleinschek, Karin (Author)
ID Steindorfer, Tobias Alexander (Author)
ID Thonhofer, Martin Simon (Author)
ID Wrodnigg, Tanja (Author)
ID Kargl, Rupert (Author) |
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| Files: |  s00706-024-03227-y.pdf (2,03 MB)
MD5: A9E85EB198D07487654B27D48DE5A3D1
 https://link.springer.com/article/10.1007/s00706-024-03227-y#Sec15
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
FS - Faculty of Mechanical Engineering
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| Abstract: | This work explores the modifcation and characterization of chitosan thin flms as a model for functionalized polysaccharide interfaces. The solid–liquid interface of oligo- and polysaccharides is crucial for various biological processes such as cell adhesion and recognition. By covalent surface modifcation of the chitosan via amide formation with diferent small molecules containing carboxylic acids, e.g. specially designed glycoside hydrolase inhibitors, interactions with biomolecules and living cells could potentially be controlled in the future. As a frst step towards this aim, three fuorescent compounds were conjugated onto nanometric chitosan thin flms. The layers were analysed by fuorescence spectroscopy, X-ray photoelectron spectroscopy, time-of-fight secondary ion mass spectrometry, and atomic force microscopy, to proof the covalent attachment of the target molecules. By this analysis, a uniform and chemically stable covalent attachment of the target molecules on the chitosan thin flms could be demonstrated under various conditions. This publication serves as a proof-of-concept-study for further biofunctionalization, pattering, and interaction studies involving polysaccharide interfaces, glycosidase inhibitors, proteins, or living cells. |
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| Keywords: | carbohydrates, fluorescence spectroscopy, amino acid, chitosan, thin flm modifcation, C-Glycosides |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 19.12.2023 |
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| Article acceptance date: | 12.06.2023 |
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| Publication date: | 03.08.2024 |
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| Publisher: | Springer |
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| Year of publishing: | 2024 |
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| Number of pages: | 11 str. |
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| PID: | 20.500.12556/DKUM-89713  |
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| UDC: | 54 |
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| ISSN on article: | 1434-4475 |
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| COBISS.SI-ID: | 203801859 |
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| DOI: | 10.1007/s00706-024-03227-y |
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| Copyright: | © The Author(s) 2024 |
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| Publication date in DKUM: | 07.08.2024 |
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| Views: | 307 |
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| Downloads: | 32 |
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| Metadata: |    |
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| Categories: | Misc.
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