| Title: | The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats |
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| Authors: | ID Semeraro, Maria Donatella (Author)
ID Almer, Gunter (Author)
ID Kaiser, Melanie (Author)
ID Zelzer, Sieglinde (Author)
ID Meinitzer, Andreas (Author)
ID Scharnagl, Hubert (Author)
ID Sedej, Simon (Author)
ID Gruber, Hans-Jürgen (Author)
ID Herrmann, Markus (Author) |
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| Files: |  Semeraro-2022-The_effects_of_long-term_moderat.pdf (1,30 MB)
MD5: B219B6D74CE1D7BA3E89DB47EC44BD27
 https://doi.org/10.1007/s00394-021-02639-4
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Purpose: Regular exercise reduces obesity and the risk of cardiovascular disease. However, health-promoting benefits of physical activity are commonly associated with increased inflammation and oxidative stress. Here, we tested whether constant moderate exercise is able to prevent or attenuate the oxidative/nitrosative stress, inflammation, and serum lipids in lean and obese rats.
Methods: Four-month-old female Sprague Dawley rats received standard or a high-fat diet. Animals were subjected to a physical activity protocol, consisting of 30 min forced treadmill exercise for 5 consecutive days per week during 10 months. Baseline and sedentary (non-exercised) rats were used as controls. Lipids, oxidized low-density lipoprotein cholesterol, nitric oxide metabolites, and pro- and anti-inflammatory markers were measured in blood collected upon euthanasia.
Results: At variance to young baseline control rats, 14-month-old animals fed normal diet had increased plasma lipid levels, including total cholesterol and triglycerides, which were further elevated in rats that consumed a high-fat diet. While treadmill exercise did not lower the amount of serum lipids in standard diet group, forced physical activity reduced non-high-density lipoprotein cholesterol in response to high-fat diet feeding. Exercised rats fed standard diet or high-fat diet had lower abundancy of nitric oxide metabolites, which coincided with increased levels of oxidized low-density lipoprotein cholesterol. Accordingly, the amount of nitric oxide metabolites correlated inversely with oxidized low-density lipoprotein cholesterol and homo-arginine. Exercise significantly reduced inflammatory cytokines in high-fat diet fed rats only.
Conclusion: Our study suggests that regular exercise alters the equilibrium between oxidative and anti-oxidative compounds and reduces pro-inflammatory cytokines. |
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| Keywords: | long-term moderate exercise, nitric oxide, nitric oxide synthase, oxidized LDL, Sprague Dawley rats, western-type diet |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 01.03.2021 |
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| Article acceptance date: | 05.07.2021 |
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| Publication date: | 28.07.2021 |
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| Publisher: | Steinkopff |
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| Year of publishing: | 2021 |
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| Number of pages: | Str. 255-268 |
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| Numbering: | Letn. 61, št. 1 |
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| PID: | 20.500.12556/DKUM-91295  |
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| UDC: | 616.1 |
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| ISSN on article: | 1436-6215 |
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| COBISS.SI-ID: | 130737155 |
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| DOI: | 10.1007/s00394-021-02639-4 |
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| Publication date in DKUM: | 10.12.2024 |
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| Views: | 114 |
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| Downloads: | 58 |
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| Metadata: |    |
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| Categories: | Misc.
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