| Title: | Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
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| Authors: | ID Šikić Pogačar, Maja (Author)
ID Langerholc, Tomaž (Author)
ID Mičetić-Turk, Dušanka (Author)
ID Smole Možina, Sonja (Author)
ID Klančnik, Anja (Author) |
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| Files: |  Pogacar-2020-Effect_of__i_Lactobacillus__i__sp.pdf (1,80 MB)
MD5: 58DAC805280E867DE434D4D0070ED99B
 https://doi.org/10.1186/s12917-020-2238-5
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
FKBV - Faculty of Agriculture and Life Sciences
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| Abstract: | Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated.
Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions.
Conclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis. |
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| Keywords: | Campylobacter jejuni, Lactobacillus spp., chicken and pig cell line, adhesion, invasion, translocation |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 03.09.2019 |
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| Article acceptance date: | 09.01.2020 |
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| Publication date: | 03.02.2020 |
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| Publisher: | BioMed Central |
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| Year of publishing: | 2020 |
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| Number of pages: | Str. 1-14 |
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| Numbering: | Letn. 16, št. članka 34 |
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| PID: | 20.500.12556/DKUM-91792  |
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| UDC: | 579.22+579.24:636.4/.5.09 |
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| ISSN on article: | 1746-6148 |
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| COBISS.SI-ID: | 512948536 |
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| DOI: | 10.1186/s12917-020-2238-5 |
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| Publication date in DKUM: | 06.02.2025 |
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| Views: | 126 |
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| Downloads: | 10 |
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| Metadata: |    |
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| Categories: | Misc.
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