| Title: | Efficient β-galactosidase immobilised on glycidyl methacrylate polyHIPE |
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| Authors: | ID Paljevac, Muzafera (Author)
ID Pečar, Darja (Author)
ID Krajnc, Peter (Author) |
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| Files: |  1-s2.0-S0032386125002502-main.pdf (7,15 MB)
MD5: 92C8BA214DFB9DA8D084D3C289DF7F40
 https://www.sciencedirect.com/science/article/pii/S0032386125002502?via%3Dihub
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) monoliths (PolyGMA) were synthesized by high internal phase emulsion (HIPE) templating and polymerisation. The porous monoliths exhibited a hierarchical porous structure with primary pores (cavities, average diameter 35 µm) interconnected by secondary pores (average diameter 5 µm). FTIR spectroscopy confirmed the chemical composition and identified characteristic functional groups of both GMA and EGDMA. The polyGMA materials were ground and sieved to obtain particles between 710 µm and 1000 µm in diameter, which were subsequently used to immobilize the enzyme β-galactosidase. Immobilization was performed using two methods, namely direct binding via epoxide groups and binding after the activation with glutaraldehyde. The glutaraldehyde method resulted in higher enzyme loading (0.43 mg of enzyme per 100 mg of polyGMA) and significantly improved catalytic activity compared to direct binding. The immobilized β-galactosidase was used for lactose hydrolysis under various conditions using both batch and flow-through reactors. Optimal activity was observed at pH 6.5 and 35°C, with kinetic parameters vmax = 0.64 mmol∙L -1 ∙min-1 and �� = 38.8 mmol∙mol-1 . Reuse tests showed stable performance over five cycles. Comparatively, non-porous polyGMA exhibited negligible enzymatic activity compared to polyHIPE supported enzyme. In addition, lactose hydrolysis was investigated in a flow-through system at different flow rates (0.5–2.5 mL∙min- ¹). The highest conversion (100%) was observed at a flow rate of 0.5 mL∙min-¹, while a higher flow rate of 2.5 mL∙min-¹ resulted in a lower conversion (approx. 35%), both at the lactose concentration of 4 g∙L - ¹. |
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| Keywords: | immobilized enzymes, glycidyl methacrylate, polyHIPE, β-galactosidase, beta-galactosidase |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 28.01.2025 |
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| Article acceptance date: | 08.03.2025 |
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| Publication date: | 14.03.2025 |
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| Publisher: | Elsevier |
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| Year of publishing: | 2025 |
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| Number of pages: | 27 str. |
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| Numbering: | Let. 325, št. članka 128264 |
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| PID: | 20.500.12556/DKUM-92178  |
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| UDC: | 66 |
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| ISSN on article: | 1873-2291 |
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| COBISS.SI-ID: | 229255427 |
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| DOI: | 10.1016/j.polymer.2025.128264 |
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| Publication date in DKUM: | 21.03.2025 |
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| Views: | 175 |
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| Downloads: | 20 |
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| Metadata: |    |
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| Categories: | Misc.
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