| Title: | First insights into the antiviral activity of chitosan-based bioactive polymers towards the bacteriophage Phi6: physicochemical characterization, inactivation potential, and inhibitory mechanisms |
|---|
| Authors: | ID Plohl, Olivija (Author)
ID Fric, Katja (Author)
ID Filipić, Arijana (Author)
ID Kogovšek, Polona (Author)
ID Tušek-Žnidarič, Magda (Author)
ID Fras Zemljič, Lidija (Author) |
|---|
| Files: |  polymers-14-03357.pdf (4,27 MB)
MD5: 45DC48B140C5523CD850B5680789783E
 https://www.mdpi.com/2073-4360/14/16/3357
|
|---|
| Language: | English |
|---|
| Work type: | Article |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | FS - Faculty of Mechanical Engineering
|
|---|
| Abstract: | The outbreak of the worrisome coronavirus disease in 2019 has caused great concern
among the global public, especially regarding the need for personal protective equipment with
applied antiviral agents to reduce the spread and transmission of the virus. Thus, in our research,
chitosan-based bioactive polymers as potential antiviral agents were first evaluated as colloidal
macromolecular solutions by elemental analysis and charge. Three different types of low and high
molecular weight chitosan (LMW Ch, HMW Ch) and a LMW Ch derivative, i.e., quaternary chitosan
(quart-LMW Ch), were used. To explore their antiviral activity for subsequent use in the form of
coatings, the macromolecular Chs dispersions were incubated with the model virus phi6 (surrogate
for SARS-CoV-2), and the success of virus inactivation was determined. Inactivation of phi6 with
some chitosan-based compounds was very successful (>6 log), and the mechanisms behind this
were explored. The changes in viral morphology after incubation were observed and the changes in
infrared bands position were determined. In addition, dynamic and electrophoretic light scattering
studies were performed to better understand the interaction between Chs and phi6. The results
allowed us to better understand the antiviral mode of action of Chs agents as a function of their
physicochemical properties. |
|---|
| Keywords: | bacteriophage phi6, bioactive polysaccharide biomaterials, chitosans, antiviral activity, dynamic and electrokinetic light scattering, charge, biointerfaces |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Submitted for review: | 20.07.2022 |
|---|
| Article acceptance date: | 11.08.2022 |
|---|
| Publication date: | 17.08.2022 |
|---|
| Publisher: | MDPI AG |
|---|
| Year of publishing: | 2022 |
|---|
| Number of pages: | str. 1-19 |
|---|
| Numbering: | Vol. 14, iss. 16 (3357) |
|---|
| PID: | 20.500.12556/DKUM-92260  |
|---|
| UDC: | 677.017:577 |
|---|
| ISSN on article: | 2073-4360 |
|---|
| COBISS.SI-ID: | 119204867 |
|---|
| DOI: | 10.3390/polym14163357 |
|---|
| Copyright: | © 2022 by the authors |
|---|
| Publication date in DKUM: | 26.03.2025 |
|---|
| Views: | 145 |
|---|
| Downloads: | 8 |
|---|
| Metadata: |    |
|---|
| Categories: | Misc.
|
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
