| Title: | A HepG2 cell-based biosensor that uses stainless steel electrodes for hepatotoxin detection |
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| Authors: | ID Rozman, Martin (Author)
ID Štukovnik, Zala (Author)
ID Sušnik, Ajda (Author)
ID Pakseresht, Amirhossein (Author)
ID Hočevar, Matej (Author)
ID Drobne, Damjana (Author)
ID Bren, Urban (Author) |
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| Files: |  biosensors-12-00160.pdf (1,87 MB)
MD5: C2C7737734A03990D2FD88C120E817EB
 https://www.mdpi.com/2079-6374/12/3/160
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | Humans are frequently exposed to environmental hepatotoxins, which can lead to liver
failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high
sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To
investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless
steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity
assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue
(CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of
aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless
steel surface was quantitatively and qualitatively examined with different microscopy techniques. A
simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2
cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using
electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the
metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples. |
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| Keywords: | HepG2 cell line, impedance biosensor, adhesion, hepatotoxins, stainless steel |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 11.02.2022 |
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| Article acceptance date: | 02.03.2022 |
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| Publication date: | 04.03.2022 |
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| Publisher: | MDPI AG |
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| Year of publishing: | 2022 |
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| Number of pages: | 1-15 |
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| Numbering: | Vol. 12, iss. 3 |
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| PID: | 20.500.12556/DKUM-92456  |
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| UDC: | 543.2/.9 |
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| ISSN on article: | 2079-6374 |
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| COBISS.SI-ID: | 99755011 |
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| DOI: | 10.3390/bios12030160 |
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| Copyright: | © 2022 by the authors |
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| Publication date in DKUM: | 10.04.2025 |
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| Views: | 179 |
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| Downloads: | 15 |
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| Metadata: |    |
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| Categories: | Misc.
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