| Title: | The effect of preconditioning strategies on the adsorption of model proteins onto screen-printed carbon electrodes |
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| Authors: | ID Romih, Tea (Author)
ID Konjević, Ivan (Author)
ID Žibret, Lea (Author)
ID Fazarinc, Ika (Author)
ID Beltram, Ajda (Author)
ID Majer, David (Author)
ID Finšgar, Matjaž (Author)
ID Hočevar, Samo B. (Author) |
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| Files: |  sensors-22-04186-v2.pdf (1,23 MB)
MD5: 0A90D278BD1CC626EECF7E5941D7CECE
 https://www.mdpi.com/1424-8220/22/11/4186
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | The preconditioning and modification of the supporting electrode surface is an essential
step in every biosensor architecture. In particular, when using screen-printed carbon electrodes (SPEs)
as inexpensive and convenient disposable sensor substrates, their somewhat lower electrochemical
(surface) reproducibility might represent a complex hurdle. Herein, we investigated the effect of
selected preconditioning strategies, such as cyclic voltammetric pretreatment, in H2SO4 and H2O2
and plasma pretreatment with a positive and negative glow discharge, which all improved the electrochemical stability of the unmodified SPEs. Furthermore, we studied the influence of preconditioning
strategies on the adsorption kinetics of the two most commonly used building blocks for biosensor
preparation, i.e., bovine serum albumin (BSA) and protein A. We observed an advantageous effect
of all the examined preconditioning strategies for the modification of SPEs with protein A, being
the most effective the negative glow discharge. On the other hand, BSA exhibited a more complex
adsorption behavior, with the negative glow discharge as the only generally beneficial preconditioning strategy providing the highest electrochemical stability. Protein A revealed a more substantial
impact on the electrochemical signal attenuation than BSA considering their same concentrations in
the modification solutions. For both BSA and protein A, we showed that the concentrations of 5 and
10 µg mL−1 already suffice for an electrochemically satisfactorily stable electrode surface after 60 min
of incubation time, except for BSA at the positive-plasma-treated electrode. |
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| Keywords: | screen-printed carbon electrode, SPE, electrochemical biosensor, adsorption, bovine serum albumin, protein A, glow discharge |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 03.05.2022 |
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| Article acceptance date: | 27.05.2022 |
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| Publication date: | 31.05.2022 |
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| Publisher: | MDPI |
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| Year of publishing: | 2022 |
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| Number of pages: | 14 str. |
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| Numbering: | Vol. 22, iss. 11 |
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| PID: | 20.500.12556/DKUM-92818  |
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| UDC: | 544.5/.6 |
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| ISSN on article: | 1424-8220 |
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| COBISS.SI-ID: | 110592003 |
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| DOI: | 10.3390/s22114186 |
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| Copyright: | © 2022 by the authors |
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| Publication date in DKUM: | 15.05.2025 |
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| Views: | 166 |
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| Downloads: | 12 |
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| Metadata: |    |
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| Categories: | Misc.
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