| Title: | Substrate-dependent variability in viability and angiogenicmarker expression among three endothelial cell subtypes : insights for artificial tissue vascularization |
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| Authors: | ID Vajda, Jernej (Author)
ID Vihar, Boštjan (Author)
ID Milojević, Marko (Author)
ID Bjelić, Dragana (Author)
ID Brečko, Amadeja (Author)
ID Maver, Uroš (Author) |
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| Files: |  Macromolecular_Bioscience_-_2025_-_Vajda_-_Substrate‐Dependent_Variability_in_Viability_and_Angiogenic_Marker_Expression.pdf (10,47 MB)
MD5: 79904CAB94EE0ABEB5FEBD9B7C1D7D06
 https://onlinelibrary.wiley.com/doi/10.1002/mabi.202500333
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | issue engineering faces the challenge of achieving effective vascularization within tissue constructs for sustained viability andoptimal function. The success of tissue-engineered constructs depends on selecting an optimal angiogenesis-stimulating ECMsubstitute material. This study compares four substrates made from three different biomacromolecules—fibrin, fibronectin,non-crosslinked, and crosslinked gelatin, and their effect on endothelial cells. Acknowledging the diverse range of endothelialcells that play a role in (micro)vascularization, human endothelial primary cells, human umbilical vein endothelial cells, andhuman microvascular endothelial cells are subjected to these materials for evaluation. Biocompatibility is assessed by measuringcell viability (Live/Dead assay), metabolic activity (alamarBlue assay), morphology (actin staining), phenotype expression(immunocytochemistry), and the production of von Willebrand factor, which promotes angiogenesis by promoting cell adhesionand migration. The results show that the use of biomaterials as culturing substrates significantly impacts the viability andmorphology of the cells. While the expression of angiogenic markers is shown to rely more on the cell lineage, the use of differentsubstrates has an impact on the expression timeline. Thus, combining cells and biomaterials in a favorable manner can be usedas a powerful tool for controlled vascularization in vitro, which requires the systematic assembly of different stimuli. |
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| Keywords: | biomacromolecules, endothelial cell types, materials, microvascularization, vascularization |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 29.05.2025 |
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| Article acceptance date: | 18.06.2025 |
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| Publication date: | 10.07.2025 |
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| Publisher: | Wiley-VCH |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 1-18 |
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| Numbering: | Vol. 25, iss. 7 |
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| PID: | 20.500.12556/DKUM-93711  |
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| UDC: | 61 |
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| ISSN on article: | 1616-5187 |
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| COBISS.SI-ID: | 242531843 |
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| DOI: | 10.1002/mabi.202500333 |
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| Copyright: | © 2025 The Author(s). |
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| Publication date in DKUM: | 22.07.2025 |
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| Views: | 150 |
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| Downloads: | 23 |
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| Metadata: |    |
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| Categories: | Misc.
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