| Title: | Siderophore production, diversity of siderophore receptors and associations with virulence-associated genes, phylogroups and bacteriocin production in Escherichia coli strains isolated from humans, animals and organic fertilizers |
|---|
| Authors: | ID Kuznetsova, Marina V. (Author)
ID Mihailovskaya, Veronika S. (Author)
ID Selivanova, Polina A. (Author)
ID Kochergina, Darja A. (Author)
ID Remezovskaya, Natalia B. (Author)
ID Starčič Erjavec, Marjanca (Author) |
|---|
| Files: |  RAZ_Kuznetsova_Marina_V._2025.pdf (1,34 MB)
MD5: 8AE0EEC05A138BE2547C91999B15CE2C
 https://doi.org/10.3390/microbiolres16020050
|
|---|
| Language: | English |
|---|
| Work type: | Scientific work |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | FNM - Faculty of Natural Sciences and Mathematics
|
|---|
| Abstract: | Iron uptake plays an important role in the persistence of Escherichia coli in the host and for its survival in the environment, and it is known that E. coli has a variety of siderophore systems for iron uptake. We investigated the ability to produce siderophores, the genetic diversity of the siderophores and their correlation with virulence-associated genes (VAGs), phylogroups and bacteriocin production in E. coli strains isolated from different sources: uropathogenic E. coli (UPEC) from urine of patients with urinary tract infections, avian pathogenic E. coli (APEC) from organs of birds with signs of colibacillosis, fecal E. coli (FEC) from feces of healthy cattle and E. coli from organic fertilizers based on poultry and cattle manure (OFEC). A high variability in siderophore production was found among the UPEC strains studied, while the OFEC strains showed the highest siderophore production among all groups. Genes for aerobactin and yersiniabactin receptors were most frequently found in the UPEC strain, followed by the APEC, FEC and OFEC strains. The greatest diversity of siderophore receptors was found in the APEC strain. We also found that iutA-positive E. coli isolated from animals contained more VAGs than iutA-negative strains. The profiles of the siderophore genes of APEC and OFEC from poultry manure were very similar, indicating that APEC can be transmitted via organic fertilizers, suggesting that poultry manure is an environmental risk. The data obtained complement the information on the prevalence of siderophore producers and contribute to our knowledge on the biodiversity of E. coli pathotypes. |
|---|
| Keywords: | siderophore, Escherichia coli, virulence-associated genes, phylogenetic group, bacteriocin, microcin |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Submitted for review: | 30.12.2024 |
|---|
| Article acceptance date: | 11.02.2025 |
|---|
| Publication date: | 17.02.2025 |
|---|
| Publisher: | MDPI |
|---|
| Year of publishing: | 2025 |
|---|
| Number of pages: | 14 str. |
|---|
| Numbering: | Letn. 16, št. 2, št. članka 50 |
|---|
| PID: | 20.500.12556/DKUM-93765  |
|---|
| UDC: | 579.2 |
|---|
| ISSN on article: | 2036-7481 |
|---|
| COBISS.SI-ID: | 227345923 |
|---|
| Publication date in DKUM: | 18.07.2025 |
|---|
| Views: | 127 |
|---|
| Downloads: | 5 |
|---|
| Metadata: |    |
|---|
| Categories: | Misc.
|
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
