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Title:Anatomske in ekofiziološke lastnosti prezimnih listov škrlatnordeče mrtve koprive (Lamium purpureum L.) v stresnih habitatih
Authors:ID Frešer, Marisa (Author)
ID Šajna, Nina (Mentor) More about this mentor... New window
Files:.pdf MAG_Freser_Marisa_2026.pdf (2,83 MB)
MD5: 997D4A57463119CC5CA8175E1D9C93C2
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Za zagotovitev preživetja lahko nekatere rastline med drugim ohranijo funkcionalne liste skozi celotno zimsko obdobje. To so prezimni listi, ki so izpostavljeni številnim abiotskim dejavnikom, vključno z nizkimi temperaturami, visoko intenziteto svetlobe pozimi in zmanjšano dostopnostjo vode. Kljub temu vzdržujejo funkcionalnost fotosinteznega aparata, s čimer so povezane fiziološke in strukturne lastnosti listov. V okviru raziskave smo omenjene lastnosti proučili pri prezimnih listih škrlatnordeče mrtve koprive in jih primerjali med rastiščema ter mesecema. Fiziološko stanje smo ovrednotili z meritvami fluorescence klorofila a in vrednostmi SPAD, anatomske značilnosti pa z analizo prečnih rezov shranjenih listov in epidermalnih odtisov svežih listov (le pri rastlinah ob njivi v decembru). Preverili smo še povezanost izbranih fluorescenčnih parametrov z vrednostmi SPAD in izbranimi okoljskimi dejavniki. Podatki so bili statistično obdelani v programu RStudio. Med rastlinami ob njivi in ob cesti v marcu pri fizioloških parametrih nismo ugotovili statistično značilnih razlik, le eno na anatomski ravni, pri širini celic povrhnjice. Pri primerjavi rastlin ob njivi med marcem in decembrom so bile razlike izrazitejše predvsem na fiziološki ravni, medtem ko na anatomski niso ohranile statistične značilnosti. Vrednosti SPAD so bile statistično značilno povezane le s temotnim Fv/Fm v dveh skupinah, pri čemer je bil delež pojasnjene variabilnosti majhen. Izbrani fluorescenčni parametri niso bili statistično značilno povezani s svetlobnimi razmerami v nobeni od treh obravnavanih skupin, pri rastlinah ob njivi v decembru pa tudi ne s temperaturo. V slednji skupini sta bili svetloba in temperatura pozitivno povezani. S pomočjo anatomskih analiz smo potrdili bifacialno zgradbo listov in prisotnost nežleznih ter žleznih (peltatnih, kapitatnih) trihomov. Ugotovili smo, da so listne reže prisotne na obeh listnih površinah (amfistomatni listi). Epidermalni odtisi so pokazali izrazite razlike med zgornjo in spodnjo listno površino ter nasproten vzorec med gostoto in velikostjo listnih rež. Rezultati kažejo, da imajo prezimni listi škrlatnordeče mrtve koprive razmeroma podobno anatomsko zgradbo med preučevanima rastiščema in mesecema. Fiziološko stanje se je medtem izraziteje razlikovalo med marcem in decembrom, ne pa tudi med rastiščema. Ugotovljenih fluorescenčnih odzivov na podlagi rezultatov ni bilo mogoče ustrezno pojasniti z vrednostmi SPAD in okoljskimi dejavniki.
Keywords:Lamium purpureum, prezimni listi, fiziološke lastnosti, fluorescenca klorofila a, SPAD, anatomske lastnosti, listne reže, trihomi, epidermalni odtis
Place of publishing:Maribor
Publisher:[M. Frešer]
Year of publishing:2026
Number of pages:IX, 73 str.
PID:20.500.12556/DKUM-99892 New window
UDC:582.929.4:581.4:581.5(043.2)
COBISS.SI-ID:292648195 New window
Publication date in DKUM:28.09.2026
Views:10
Downloads:0
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:28.08.2026

Secondary language

Language:English
Title:Anatomical and ecophysiological characteristics of overwintering leaves of purple dead-nettle (Lamium purpureum L.) in stressful habitats
Abstract:To ensure survival, some plants can retain functional leaves throughout the winter. These overwintering leaves are exposed to various abiotic factors, including low temperatures, high winter light intensity, and reduced water availability. Despite these conditions, they maintain the functionality of the photosynthetic apparatus, and this process is associated with the physiological and structural characteristics of leaves. In this study, these characteristics were investigated in overwintering leaves of Lamium purpureum and compared across sampling sites and months. Physiological state was assessed using chlorophyll a fluorescence measurements and SPAD values. Anatomical characteristics were examined using cross-sections of preserved leaves and epidermal impressions of fresh leaves. Impressions were obtained only from leaves of plants growing beside the field in December. The relationships between selected fluorescence parameters, SPAD values, and selected environmental factors were also examined. Statistical analyses were performed using RStudio. No statistically significant differences in physiological parameters were found between plants growing beside the field and the road in March, while only one anatomical difference was detected in epidermal cell width. When plants growing beside the field were compared between March and December, differences were more pronounced at the physiological level, whereas anatomical differences did not remain statistically significant. SPAD values were significantly associated only with dark-adapted Fv/Fm in two groups, although the proportion of explained variance was small. The selected fluorescence parameters were not significantly associated with light conditions in any of the three groups, nor with temperature in plants growing beside the field in December. In this group, a positive association between light and temperature was observed. Anatomical analyses confirmed a bifacial leaf structure, amphistomatous leaves, and the presence of non-glandular, peltate glandular, and capitate glandular trichomes. Epidermal impressions showed pronounced differences between the adaxial and abaxial leaf surfaces and an inverse pattern between stomatal density and stomatal size. The results indicate that the overwintering leaves of Lamium purpureum have a relatively similar anatomical structure across studied sites and months. In contrast, physiological state differed more markedly between March and December, but not between sites. Based on the obtained results, it was not possible to adequately explain the observed fluorescence responses by SPAD values or the examined environmental factors.
Keywords:Lamium purpureum, overwintering leaves, physiological characteristics, chlorophyll a fluorescence, SPAD, anatomical characteristics, stomata, trichomes, epidermal impression


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