| Title: | Covalent binding of heparin to functionalized PET materials for improved haemocompatibility |
|---|
| Authors: | ID Kolar, Metod (Author)
ID Mozetič, Miran (Author)
ID Stana-Kleinschek, Karin (Author)
ID Fröhlich, Mirjam (Author)
ID Turk, Boris (Author)
ID Vesel, Alenka (Author) |
|---|
| Files: |  Materials_2015_Kolar_et_al._Covalent_Binding_of_Heparin_to_Functionalized_PET_Materials_for_Improved_Haemocompatibility.pdf (1,70 MB)
MD5: E5BD212AD7F1702BB9D0D2977D1402D0
 http://www.mdpi.com/1996-1944/8/4/1526/
|
|---|
| Language: | English |
|---|
| Work type: | Scientific work |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | FS - Faculty of Mechanical Engineering
|
|---|
| Abstract: | The hemocompatibility of vascular grafts made from poly(ethylene terephthalate) (PET) is insufficient due to the rapid adhesion and activation of blood platelets that occur upon incubation with whole blood. PET polymer was treated with NHx radicals created by passing ammonia through gaseous plasma formed by a microwave discharge, which allowed for functionalization with amino groups. X-ray photoelectron spectroscopy characterization using derivatization with 4-chlorobenzaldehyde indicated that approximately 4% of the –NH2 groups were associated with the PET surface after treatment with the gaseous radicals. The functionalized polymers were coated with an ultra-thin layer of heparin and incubated with fresh blood. The free-hemoglobin technique, which is based on the haemolysis of erythrocytes, indicated improved hemocompatibility, which was confirmed by imaging the samples using confocal optical microscopy. A significant decrease in number of adhered platelets was observed on such samples. Proliferation of both human umbilical vein endothelial cells and human microvascular endothelial cells was enhanced on treated polymers, especially after a few hours of cell seeding. Thus, the technique represents a promising substitute for wet-chemical modification of PET materials prior to coating with heparin. |
|---|
| Keywords: | poly(ethylene terephthalate), vascular graft, biocompatibility, heparin, plasma, functionalization, haemolysis, platelet adhesion, endothelization |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Year of publishing: | 2015 |
|---|
| Number of pages: | str. 1526-1544 |
|---|
| Numbering: | Letn. 8, št. 4 |
|---|
| PID: | 20.500.12556/DKUM-60193  |
|---|
| ISSN: | 1996-1944 |
|---|
| UDC: | 577 |
|---|
| ISSN on article: | 1996-1944 |
|---|
| COBISS.SI-ID: | 28469543 |
|---|
| DOI: | 10.3390/ma8041526 |
|---|
| NUK URN: | URN:SI:UM:DK:VY41XAFZ |
|---|
| Publication date in DKUM: | 21.06.2017 |
|---|
| Views: | 1508 |
|---|
| Downloads: | 388 |
|---|
| Metadata: |    |
|---|
| Categories: | Misc.
|
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
